Composite

Part:BBa_K209446:Design

Designed by: Katja Kolar   Group: iGEM09_UCSF   (2009-10-05)


pEF1-SSFYFP-hM4D-ITSN


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3694
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3694
    Illegal NheI site found at 2081
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3694
    Illegal BglII site found at 128
    Illegal BglII site found at 3814
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3694
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3694
    Illegal PstI site found at 346
    Illegal PstI site found at 1050
    Illegal PstI site found at 1845
    Illegal NgoMIV site found at 1952
    Illegal NgoMIV site found at 2465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 84


Design Notes

Cloned into pEF5/FRT/V5-DEST.


Source

References